Freezing and Thawing System - CRFT
CRFT – because in cryopreservation, every degree counts.
The CRFT system makes biological cryopreservation more precise, controlled, and reproducible—entirely without the use of liquid nitrogen or other cryogens. This innovative system enables exact regulation of freezing and thawing processes, laying the foundation for reliable results in biological research.
Every detail matters, especially during the critical phase of nucleation (seeding). CRFT provides precise control over the entire process, creating optimal conditions for cell recovery after thawing.
Whether it is stem cells, mammalian cells, spermatozoa, antibodies, tissue sections, or rodent organs, CRFT opens up a wide range of possibilities for demanding research applications.
Request a quoteFreezing and Thawing System CRFT
CRFT is an innovative system for controlled cryopreservation that does not require liquid nitrogen or other cryogens. The CRFT system brings accuracy, precision, and reproducibility to biological cryopreservation. The device is ideally suited for research into the cryopreservation of a wide range of materials, including stem cells, mammalian cells, spermatozoa, antibodies, tissue sections, and rodent organs.
Freezing and thawing rates are precisely controlled, ensuring accuracy and reproducibility throughout the entire process—particularly during the critical nucleation (seeding) phase. This enables optimal cell recovery after thawing.
Operation is simple and can be performed with or without a PC; data can be recorded using PC software, and profiles are displayed directly on the PC screen. Various freezing profiles are available via a drop-down menu, and custom profiles can be created using the software's editing function.
Specifications
- Precise and reproducible control of cooling rates and sample temperatures
- Focus on maximizing cell survival rates
- Controlled and customizable freezing and thawing
- Linear and non-linear freezing profiles
- No need for liquid nitrogen, alcohol, or other cryogens
- Interchangeable inserts for high flexibility (optional; see p. 2)
- Free software for creating, monitoring, exporting, and documenting temperature profiles
- Easy operation; in-situ nucleation (seeding) of samples possible
- Low operating costs: estimated at 1% of the cost of a liquid nitrogen-based system; powered by a 24V power supply
- Temperature range from -100°C to +30°C*
Appliciations
The CRFT is highly versatile and can be used for the cryopreservation research of a wide range of samples in cryovials, straws, bags, microplates and Matrix-96-well block plates in the following areas:
- Stem cell research
- Clinical and research samples, e.g. lymphocytes and tissue cell lines in conventional cryovials
- Various mammalian cells including cardiomyocytes, adipose, liver and muscle
- Cord blood derived stem cells
- Adherent cells and stem cells in microplates
- Cell suspensions in numbered/barcoded arrays
- System integrations
- Suitable for applications in veterinarian IVF
| Dimensions (W x D x H | 345 x 290 x 380 (without lid) / 460 (with lid) |
| Weight | 5 kg |
| Max. plate temperature | +30°C |
| Min. plate temperature | -100°C* |
| Temperature accuracy | +/- 0,5°C |
| Temperature stability | +/- 0,1°C |
| Temperature uniformity | +/- 1,0°C Ref |
| Max. cooling rate | 10°C/min |
| Max. thawing rate | 10°C/min from -100°C |
| Max. temperature stabilisation time | 5 min |
| Power supply | 100-240V, 50-60 Hz; 24 V DC unit supply |
*Minimum head temperature achieved with sealed lid in place. Temperatures specified are applicable to CRFT head (sample temperatures may vary).
