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Densitometer DEN-1

Measurement of Cell Suspension Turbidity

Densitometers are designed to measure cell suspension turbidity in the following range: 0.3-5.0 McFarland units (0 - 180 × 107 cells/ml).

They allow the measurement of solution turbidity in a wide range (up to 15.0 Mcfarland units).
However, it should be noted that the standard deviation values increase in the process.

The principle of operation is based on the measurement of optical density with digital representation of the results in McFarland units.

 

0.00 – 15.00 McF

Part Number: 103.3500

DEN-1 Densitometer

Applications

  • Measurement of cell concentration (bacterial, yeast cells) during fermentation process.
  • Determination of microorganism sensitivity to antibiotics.
  • Microorganism identification using various test-systems.
  • Measurement of absorption at the definite wavelength.
  • Quantitative estimation of concentration of colour solution, absorbing green light.

The operation principle is based on measurement of optical density with digital presentation of  results in McFarland units.
The unit is calibrated at the factory (for operation with 16 mm diameter glass tubes) and keeps calibration without power supply.
However, if necessary it is possible to calibrate the unit by 2–6 points in 0.0–6.0 McFarland unit range.

We recommend to use Biosan standards to ensure full reliability.
It is acceptable to use other commercial as well as self prepared standards (e.g. BaSO4). Possibility to restore factory calibration settings.

Optional Accessories for DEN-1

Following calibration kits are available:

  • CKG16 for glass tubes with diameter 16 mm, set of 0.5; 1.0; 2.0; 3.0; 4.0 McFarland Turbidity Standards (latex particles);
  • CKG1802 for glass tubes with diameter 18 mm, set of 0.5; 1.0; 2.0; 3.0; 4.0; 5.0 McFarland Turbidity Standards (BaSO4).

Glass Sample Tubes
Without lid (16х100x0.8mm), 78 pcs. Fits DEN-1, DEN-1B factory calibrated

CKG16
For glass tubes with diameter 16 mm, set of 0.5; 1.0; 2.0; 3.0; 4.0 McFarland Turbidity Standards (latex particles).

Calibration Kit
McFarland turbidity standards, Ø18mm

Measurement range0.00 – 15.00 McF

Measurement wavelength (λ)

λ = 565 ±15 nm

Display resolution

0.1 McF

Light sourceLED

Accuracy (0.3 - 5.0 McF)

± 3%

Measurement time

1 s

Sample volume

not less than 2 ml

Tube external diameter

16 mm using A-16 adapter) or 18 mm (without adapter)

Possibility to restore factory calibration settings

Yes

Display

LCD

Overall dimensions (W×D×H)

165 × 115 × 75 mm

Weight

0.7 kg

Input current/power consumption

12 V, 7 mA / 0.1 W

External power supply

Input AC 100-240 V, 50/60 Hz;
Output DC 12 V

Standard set

External power supply
A-16 Adapter

Can I use tubes of different diameter?

Yes. Glass and transparent plastic tubes (12 (only on DEN-1B), 16 or 18 mm in external diameter) can be used for work with DEN densitometers. A-12 (optional), A-16 adapter (included) must be used for work with tubes which are 12mm, 16 mm in external diameter.

Note! The unit must be calibrated every time a tube type (e.g. with different ext. diameter, bottom shape or different material) is changed.

Can standards prepared in the laboratory be used for calibration?

Yes. Both commercial standards and standards prepared in the laboratory can be used for calibration.

For what type of tubes is DEN-1/DEN-1B precalibrated at the factory?

From December 2015 factory calibration is for work with glass tubes with an external dimeter of 16 mm.

How to caibrate DEN-1/DEN-1B?

Calibration procedure is described in the user manual p.3.4. See user manual.

How to restore factory calibration settings?

Procedure of restoration of factory calibration settings is described in the user manual p 3.5. See user manual.

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