Real-Time Cycler - RotorGene Q

Real-Time Cycler RotorGene Q

Specifications

• Excellent thermal and optical performance thanks to the rotational format
• An unmatched optical range from UV to infrared wavelengths
• Cutting-edge analysis with user-friendly software
• Low maintenance and maximum convenience thanks to a robust design
• High performance in a wide range of applications

Typical Applications

• Gene expression analysis
• Pathogen detection
• DNA methylation analysis
• Genotyping and gene scanning
• miRNA research

Minimal Maintenance Effort
The Rotor-Gene Q is designed for minimal maintenance:

• Highly stable LEDs (no lamp or laser replacement, no gradual light source power loss)
• No optical calibration required during installation or transport
• No sample block to clean
• No condensation or bubbling in reactions due to rotation

Rotary Design
The unique centrifugal rotation design of the Rotor-Gene Q makes it a precise and versatile real-time PCR cycler.
Each tube rotates in a chamber with moving air, keeping all samples at exactly the same temperature during rapid temperature cycling. Detection is consistent for each tube.
When each tube is aligned with the detection optics, the sample is illuminated, and the fluorescent signal is quickly captured by a single, short optical path.
This thermal and optical uniformity results in sensitive, precise, and fast real-time PCR analysis. It also eliminates sample-to-sample variations and edge effects, which are unavoidable with conventional block-based instruments due to temperature gradients across the block and multiple, complex optical paths.

Temperature
Thermal uniformity, equilibration time uniformity (the time each well needs to reach a set temperature), and temperature accuracy are the thermal variables important for real-time analysis. Thanks to its rotary design, the RotorGene Q offers very good thermal uniformity.
Thermal accuracy, however, depends on correct instrument calibration, and this is where the RotorGene Q excels:
To ensure the repeatability and transferability of validated protocols, the RotorGene Q uses an
OTV^TM (Optical Temperature Verification) rotor that automates verification tests.
A printable report documents each test. In the unlikely event that the instrument needs recalibration, this is also automatically adjusted and documented. Each test takes only a few minutes and can be repeated at any time and as often as required.

Optics
The RotorGene Q uses a separate high-power light-emitting diode (LED) as the excitation source for each channel. Each LED delivers consistent power and is guaranteed for life.
Optical calibration is a topic few vendors like to discuss. The RotorGene requires no optical calibration because the identical optical path reaches all samples. Other systems require complex optical alignment and calibration.
For the same reasons, the RotorGene Q also does not require a reference dye (like ROX™). To verify performance, the OTV Rotor (see above) checks the optical system during every use. This ensures that your system is always performing at its peak.

Security
Data security is often neglected, but is essential for many laboratories. Each RotorGene result file receives a digital signature, which, if valid, ensures that the test data has not been manually altered or otherwise compromised. You also have the option to designate individual users as analyzer, operator, or administrator, each with different access rights.
Permissions are integrated into the central Windows security modules, simplifying management and integration into your current operational processes. Additionally, audit logs are stored with the run data to document changes to test files, their time of modification, and the author.

HRM – High Resolution Melt
The RotorGene Q features a specially tuned high-intensity optical channel, high-speed data acquisition (up to 1000 data acquisition points per °C transition), extreme thermal resolution (±0.02°C), and dedicated HRM analysis software.
HRM characterizes nucleic acid samples based on their dissociation (melting) behavior. Samples can 
be differentiated based on their sequence, length, GC content, or strand complementarity.
Even single-base changes such as SNPs (single nucleotide polymorphisms) can be easily identified.

Real-Time Quantification
The RotorGene Q is a versatile real-time analyzer. With it you can work with dyes covering the entire spectrum, from infra-red to UV and up to 6-plex capability.