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UVC Crosslinker CL3000

UV Crosslinker CL3000

The UVP Crosslinker is a microprocessor controlled UV irradiation system dedicated to nucleic acid linking to membranes for Southern, Northern, Dot and Slot Blot applications.

It can also be used for UV disinfection and for elimination of PCR contaminations.

Disinfection of FFP2-Masks

Part Number: 110.4305

Shortwave UV (UVC) as a Disinfectant

Ultraviolet light is considered an ancillary disinfectant to chemical disinfectants in the healthcare environment. Our high-precision CL-3000 crosslinker is capable of producing a cumulative dosage of 1 J/cm2 , this is considered well beyond the requirement needed for disinfection. The CL-3000 provides nearuniform UVC light with a ~10% coefficient of variation and less than 2 mJ/cm2 difference between the highest and lowest reading. Most importantly, the CL-3000 has real-time monitoring of UVC dosage to assure reproducible dosing irrespective of space and time.

Application Example
Up to 6 masks can be disinfected with this device in about 5 minutes, including turning the mask over.
The masks should be checked for wear and tear after each disinfection cycle.

Wavelength254 nm
Bulb6 x 8 Watt
Energy0000.1 - 9999.9 mJ/cm2 (0 - 10 J/cm2)
Time000:01 - 999:59 mmm:ss (>300 J/cm2)
Temperature15 °C - 35 °C
Humidity70% Non-Condensing
Altitudeup to 3.000 m (9,842 ft)
Sound Level≤ 50 dba
Housing Surface Temp≤ 30 °C
Startup Time< 1 sec
External Dimensions (L x W x H)41 x 40 x 26.5 cm
Internal Dimensions (L x W x H)35 x 27 x 16 cm
Weight6.8 kg
Operating Power230 V, 50/60 Hz
CertificationsCE, RoHS (CSA in process)

Mid-Range Wavelength: 302 nm (CL-3000M) and Long-Range Wavelength: 365 nm (CL-3000L) UVP Crosslinkers are available to order, but are not applicable to this application note.

1. What is UVGI?

UVGI stands for “ultraviolet germicidal irradiation”. Ultraviolet light is damaging to DNA/RNA molecules. A damaged DNA/RNA molecule cannot be used by the cell for normal cellular processes. A cell that cannot use its copies of DNA/RNA will not be able to function. Utilizing this fact, UVGI is popular for use as a general laboratory disinfection protocol. It is also a popular technique to eliminate DNA/RNA contamination (amplicons) in PCR experiments. UVGI uses light emitted at 254 nm, a high energy wavelength that damages these critical nucleic acids.

2. How is UVGI used to disinfect masks?

There are numerous studies that have investigated this strategy over the past several years, which have most recently been implemented in response to the COVID-19 pandemic. Both the CDC and other groups around the country have made recommendations for N95 mask reuse and the general disinfection of services using UVGI. In general, since UVC light is known to damage critical DNA/RNA molecules in microorganisms, UVGI techniques may be used on materials that aren’t ideal for chemical disinfection. In this case, using alcohol or bleach on a mask isn’t practical or economical and has been demonstrated to affect filtration performance. Therefore, UVC light may be used since it only requires a basic power outlet to operate.

3. Has UVGI been used in hospitals before?

UVGI is a routine application for disinfecting hospital rooms surfaces and circulating air.

4. How does the CL-3000 cross-linker replicate the recommended protocols for mask disinfection?

The current recommendation is to deliver a 1 J/cm2 dose to each side of the mask8,11. The Crosslinker CL-3000 has a dose monitor inside the unit that determines the level of UVGI energy delivered to the mask. The unit can be set up with 10 different protocols, to deliver a range of UV doses for disinfection applications. The internal monitor considers any variations in intensity due to temperature or age of the bulbs, so the exact amount of energy is delivered each and every time.

5. How is the UVGI generated in the cross-linker? How long do the bulbs last?

The Crosslinker CL-3000 uses a proprietary design to power six 254 nm UVGI bulbs. The bulbs have a lifetime of approximately 5000 to 6000 hours each. Replacement bulbs can be ordered from LTF Labortechnik, and easily installed into the CL-3000.

6. Are there other applications for the CL-3000 UV cross-linker?

Yes, there are numerous applications outside of disinfection for the CL-3000, including the elimination of amplicons during PCR sample preparation, attaching nucleic acids to surfaces such as nylon membranes in a southern blot and developing cross-linked matrices. The CL-3000 also has applications in fabricating microarrays, working with microfluidics, cross-linking RNA and protein, and finally skin cancer research. The CL-3000 can be used in any application requiring UV light.

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