Low cost and very compact pipetting machine for PCR and Real-Time PCR. Used and top refurbished. The CAS-1200 is configured to automatically prepare DNA master mix and load up to 96 samples with high speed and precision. Level sensing is a standard feature that ensures reproducible volume delivery for all reagents. These instruments are completely renewed by our technical department and are in a very good condition.
*subject to prior sale
Part Number: 112.3000
Real-Time PCR applications
With the advent of real-time PCR it is now even more critical that pipetting steps are tightly controlled. Unfortunately pipetting real-time PCR protocols manually is both laborious and prone to error. Volumetric errors that are tolerated by gel based analyses systems show up as sample-to-sample deviation in real-time systems. The pipetting precision can be determined by the standard deviation of replicate samples that are used to create a standard curve. Sample preparation with the CAS-1200 eliminates these errors so less replicates need to be run and the need to re-run assays is reduced.
From the graphs it is noted that all replicates are identical resulting in an R value for the standard curve of R=0.99960 samples were prepared on the CAS-1200 in under 15 minutes.
Standard PCR Software Module
The standard software has been designed specifically for the preparation of regular and real-time PCR assays. This software can be upgraded and the table configuration changed as new software modules are developed for other applications.
|Pipetting Head||Single Channel, 20 - 150 µl/sec (software controlled)|
CV < 1% >5 µl (dry-well transfer),
CV < 5% 2 - 5 µl (wet-well-transfer)
|Tip Volume||1,0 - 200,0 µl|
|Standard Tips||96-racked Capp™ compatible 50 µl and/or 200 µl low retention tips (LLS only)|
|Conductive Tips (optional)||96-racked, compatible 50 μL and/or 200 μL conductive tips (LLS only)|
|LLT-Liquid Level Tracking||Software to track liquid levels in every tube during normal operation. Standard on all models|
|LLS-Liquid Level Sensing (optional)||Liquid level sensing down to 10 μL in 0.2 mL PCR tubes. Liquid levels are detected at every pipetting|
event in real-time. For conductive tips only. LLS is only available with
initial instrument purchase.
|Tip Waste Handling||All tips ejected externally; no waste accumulation within deck area.|
High-capacity covered waste bag or container can be fitted at user discretion.
|UV sterilization (optional)||UV light system for deck sterilization within closed hood. Available only with initial purchase.|
|HEPA Filter (optional)||Dual-cartridge 3M Air-Mate™ HEPA (High Efficiency Particulate Air) filter system provides positive|
pressure clean air within the closed hood. Can be purchased separately.
|Hood||Integrated strut-supported tinted acrylic hood for access on 3 sides.|
|Dimensions||H (hood closed) 390 mm (15.4”); 420 mm (16.5”) UV light option fitted|
H (hood open) 770 mm (30.3”)
W 490 mm (19.3”)
D 580 mm (23”); 640 mm (25.6”) including cables
|Weight||30 kg (55 lbs) standard configuration|
|Electrical||100–120 VAC @ 4 Amp (50/60 Hz), 200–240 VAC @ 2 Amp (50/60 Hz)|
|Table Capacity||6 x Microplates (SBS standard size, holds any combination of tips/plates/tubes/capillaries etc)|
1 x Mastermix blocks (hold 1.5 mL and/or 5 mL tubes, other combinations available)
1 x Reagent block (standard holds 8 x 1.5 mL tapered tubes, 8 x 1.7 mL straight-sided tubes
16 x 200 μL PCR tubes, other combinations available)
|Startup Consumables (included)||10 racks of 96 x 50 μL tips, 10 racks of 96 x 200 μL tips, 25 x 5 mL tubes, 200 x 0.2 mL flat cap PCR tubes|
|Startup Accessories (included)||2 x Loading Blocks; 96 x 0.2 mL PCR tubes (P/N 0007)|
1 x Flip-Cap Loading Block for 32 x 1.5 mL tubes (P/N 2627)
1 x Flip-Cap Loading Block Tube Lifter for 32 x 1.5 mL tubes (P/N 2628)
1 x Reagent Block (P/N 0004)
2 x Mastermix Blocks (P/N 0002, P/N 0003)
4 x Aluminum Tip Racks (P/N 0060) for tips (included with LLS option only)
1x Pipettor head maintenance kit (P/N 0644)
|Minimum Computer||Pentium® IV, 2.6 GHz (desktop) Pentium® M 1.6GHz (laptop) or higher, 256 MB RAM|
(512 MB recommended), 40 GB HDD, USB or Serial RS-232 Port, DVD/CDROM, 1024 x 768 monitor
(speakers recommended), Windows XP® OS
|Software||Supplied, with unlimited user license. Free upgrades by web download.|
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