The XpressDNA Blood kit provides a simple and quick system to isolate high-quality genomic DNA from blood, whether fresh or aged. The XpressDNA™ technology provides an easy and flexible magnetic nanoparticles based method.
The exact part number can be found in the table under Technical Data.
Part Number: LTF00247
DNA extracted using XpressDNA™ Blood kit is amenable for all downstream applications such as PCR, Real Time-PCR, restriction digestion and sequencing.
The XpressDNA™ Blood kit provides a simple and quick system to isolate high quality genomic DNA from blood, whether fresh or aged. The XpressDNA™ technology provides easy and flexible magnetic nanoparticles based method that allows the extraction of high quantities of pure DNA as evident from the 260/280 ratio and the agarose gel electrophoresis.
Quality of DNA extracted using the XpressDNATM Blood kit by monitoring the 260/280 ratio. Data shown below were collected every alternate day and span over 2 months showing that the quality of DNA remains high when extracted using this kit.
Total number of samples extracted in 66 days = 120+
Average recovery for 400 µl blood: -12µg
Average 260/280 ratio = 1.80
Average 260/230 ratio = 2.01
High yields of pure DNA from blood. Genomic DNA was extracted from 400 µl of fresh blood using XpressDNATM Blood kit. Lane 1: 1kb ladder, Lanes 2 to 5: DNA extracted using XpressDNATM blood kit.
The MagnaStand consists of a base station and a tube holder moulded together. The tube holder can accommodate 12 microcentrifuge tubes. The base of the MagnaStand contains six rare-earth magnets, that comes in contact with the microcentrifuge tube when in-use.
The XpressDNA Blood Mini Kit is available to process 25 and 50 samples.
|Components||25 Reactions||50 Reactions|
|Lysis Buffer-1||52 ml||104 ml|
|Lysis Buffer-2||15 ml||30 ml|
|Solution A||1.75 ml||3.5 ml|
|MagNa Mix||10 ml||20 ml|
|Wash Buffer 1||10 ml||20 ml|
|Wash Buffer 2||8 ml||16 ml|
|Proteinase K (Lyophilized)||10 mg/0.5 ml||20 mg/ml|
|Proteinase K Buffer||0.5 ml||1 ml|
|Quick Reference Card||1||1|
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