Manual Nukleotide Extraction

XpressDNA Blood Mini Kit

Mini Kits for manual nucleotide extraction

The XpressDNA Blood kit provides a simple and quick system to isolate high-quality genomic DNA from blood, whether fresh or aged. The XpressDNA™ technology provides an easy and flexible magnetic nanoparticles based method.

The exact part number can be found in the table under Technical Data.

25 or 50 reactions

Part Number: LTF00247

DNA extracted using XpressDNA™ Blood kit is amenable for all downstream applications such as PCR, Real Time-PCR, restriction digestion and sequencing.

The XpressDNA™ Blood kit provides a simple and quick system to isolate high quality genomic DNA from blood, whether fresh or aged. The XpressDNA™ technology provides easy and flexible magnetic nanoparticles based method that allows the extraction of high quantities of pure DNA as evident from the 260/280 ratio and the agarose gel electrophoresis.

Quality of DNA extracted using the XpressDNATM Blood kit by monitoring the 260/280 ratio. Data shown below were collected every alternate day and span over 2 months showing that the quality of DNA remains high when extracted using this kit.

Statistics:
Total number of samples extracted in 66 days = 120+
Average recovery for 400 µl blood: -12µg
Average 260/280 ratio = 1.80
Average 260/230 ratio = 2.01

High yields of pure DNA from blood. Genomic DNA was extracted from 400 µl of fresh blood using XpressDNATM Blood kit. Lane 1: 1kb ladder, Lanes 2 to 5: DNA extracted using XpressDNATM blood kit.

 

Accessory MagnaStand

The MagnaStand consists of a base station and a tube holder moulded together. The tube holder can accommodate 12 microcentrifuge tubes. The base of the MagnaStand contains six rare-earth magnets, that comes in contact with the microcentrifuge tube when in-use.   

  • Capacity: 12 microcentrifuge tubes
  • Product size: 1 each
  • Storage & Shipping: Room temperature

Kit contents

The XpressDNA Blood Mini Kit is available to process 25 and 50 samples.

Components25 Reactions50 Reactions
Part number111.0103111.0102
Lysis Buffer-152 ml104 ml
Lysis Buffer-215 ml30 ml
Solution A1.75 ml3.5 ml
MagNa Mix10 ml20 ml
Wash Buffer 110 ml20 ml
Wash Buffer 28 ml16 ml
Proteinase K (Lyophilized)10 mg/0.5 ml20 mg/ml
Proteinase K Buffer0.5 ml1 ml
Quick Reference Card11

 

MagGenome

XpressDNA Blood kit-MagGenome technologies Pvt. Ltd.

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