Im Bereich Fertigmixe finden Sie die Produkte SensiMix HRM, SensiMix One-Step Probe Mix, SensiMix Sybr Green Mix, ...

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Features:

• cDNA synthesis and subsequent polymerisation performed in a single step
• Enhances signal-to-noise ratio
• Reduces the need for reaction-to-reaction optimisation
• Eliminates formation of non-specific products furing reaction set-up
• Normalises well-to-well variation (required in some machines)
• Minimal Optimisation Required
  Most applications can be undertaken with no or minimal optimisation.
• Highly Specific
  SensiMix contains a unique blend of highly stable reverse transcriptase enzyme in addition to a heat-activated Polymerase for increased sensitivity, specificity and efficiency.
• Universal
  SensiMix One-Step is suitable for both Sybr Green and gene-specific probe analysis.
• Wide Dynamic Range
  SensiMix One-Step demonstrates high efficiency and amplification over a broad dynamic range, enabling wide differential gene expression to be analysed in the same experiment.
• Convenience
  The combination of reagents in a single tube minimises reaction set-up and contamination risk.

SensiMix One-Step uses a combination of a highly stable reverse-transcriptase and a heat-activated DNA polyerase which allows cDNA synthesis and subsequent polymerisation to be performed in a single step, using gene-specific primers and total or poly (A) RNA.

SensiMix One-Step displays unsurpassed reproducibility and has been optimised for high-sensitivity applications where the template may be limiting. RNase Inhibitor is provided to ensure high quality intact RNA template, essential for successful cDNA synthesis.

A separate Sybr Green tube is supplied for optional use with this product. For final optimisation, an additional 50 mM MgCI₂ stock solution is also supplied to enable the user to vary the concentration if desired.

2x SensiMix One-Step containing:

• Highly stable reverse-transcriptase
• Heat-activated DNA Polyersase
• Internal reference
• 99% pure dNTPs
• MgCI₂

Plus separate:

• Sybr Green I
• RNase Inhibtor
• 50 mM MgCI₂

Specification

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Features

• Enhances signal-to-noise ratio
• Reduces the need for reaction-to-reaction optimisation
• Eliminates formation of non-specific products furing reaction set-up
• Normalises well-to-well variation (required in some machines)
• Universal Kits
  Optimised for Sybr Green or gene-specific probe analysis.
• Highly Specific
  SensiMix contains a heat-activated Polymerase which prevents mispriming.
• Minimal Optimization required
  Most applications can be undertaken with no or minimal optimisation.
• Reproducibility
  SensiMix is ready-to-go in a 2x format requiring minimal pipetting steps. SensiMix is subjected to rigorous QC procedures to ensure minimal batch-to-batch variation.

SensiMix (dT)

This universal kit displays unsurpassed reproducibility and has been optimised for high-sensitivity applications where the template may be limiting. Researchers who do not wish to incorporate a dUTP/UNG step should use Rainbow SensiMix (dt.

A separate Sybr Green tube is supplied for optional use with this product. For final optimisation, an additional 50 mM MgCI₂ stock solution is also supplied to enable the user to vary the concentration if desired.

2x Reaction Buffer

SensiMix (dU)

This universal kit is suited to laboratories where the risk of cary-over contamination is greater (e.g. where DNA amplification and reaction set up occur in close proximity). Such risk can be minimised using the separate Uracil DNA Glycosylase (UNG), tube provided.

A separate Sybr Green tube is supplied for optional use with this product. For final optimisation, an additional 50 mM MgCI₂ stock solution is also supplied to enable the user to vary the concentration if desired.

2x Reaction Buffer:

• Heat-activated DNA Polymerase
• Internal reference
• 99% pure dNTPs
• MgCI₂

Plus:

• Sybr Green I
• 50 mM MgCI₂
• UNG

Specification

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