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BioScript - Ultra Stable Reverse Transcriptase

BioScript is a Monoley Murine Leukaemia Virus (MMLV) reverse transcriptase which exhibits high stability and is active at a wide range of temperatures. Unlike the wild type enzyme, BioScript also possesses low RNase H activity, which results in enhanced yields. In addition, BioScript is highly sensitive even then the amount of template is a limiting factor. BioScript is suitable for first-strand cDNA synthesis for cDNA library construction and the production of templates for real-time PCR analysis of gene expression. Without using additional DNA polymerase, second-strand cDNA synthesis can also be achieved from some mRNA templates. BioScript can be used with tottal RNA, mRNA or in vitro-transcribed RNA.

Special Features of BioScript

• Ultra-stable: no loss of activity observed when stored at room temperature for a week
• Ultra-pure (noDNase/RNase contamination)
• High yields achieved (low RNase H activity)
• Highly sensitive even with low amounts of template
• Suitable for experiments carried out at a wide range of temperatues (37° to 50°C)
• High-quality cDNA obtained
• Suitable for real-time PCR experiments
• Price-competitive

Specification