The product group "Crosslinker" contains the products Bio-Link BLX, UV CrossLinker - TransLinker, ...
The BIO-LINK BLX is a polyvalent UV 254 nm (also available 312 nm and 365 nm) irradiation system mainly dedicated for the linking of nucleic acids to membranes.
Time Saving:
To fix nucleic acids to membranes the traditional method consists of baking the membranes for two hours 80°C in a vacuum oven. The BioLink BLX requires only a few seconds UV 254 nm irradiation.
Improvement of the signal intensity:
The hybridization signal resulting from UV irradiation is 5 to 10 times higher than with traditional baking.
Reproducibility:
The BioLink BLX is equipped with a UV Energy programming system in Joules/cm². The UV Energy is permanently monitored by a microprocessor controlled photo feedback system which compensates for variation in output and tube aging. The irradiation automatically stops when the energy received matches the programmed energy. Consequently, the UV doses are perfectly reproducible whatever the UV source intensity and variation in time.
Application:
The BioLink BLX is a polyvalent UV 254 nm irradiation system mainly dedicated for the following applications in the laboratory:
- Crosslinking of DNA and RNA to nylon or nitrocellulose membranes for Southern, Northern, dot and slot blots procedures.
- Nicking of ethidium bromide stained DNA in agarose gels
- Partial restriction endonuclease digestion by formating of thymine dimers.
- RecA mutation screening
- Elimination of PCR contamination
- UV sterilisation, UV curing of polymers
Ultraviolet Crosslinkers for DNA Crosslinking
Choose between three wavelengths:
254 nm, 302 nm und 365 nm.
Model CL-1000
- Compact design:
Exterior (D x W x H): 248 x 396 x 222 mm
Interior (D x W x H): 305 x 254 x 127 mm - 5 mal 8 Watt Bipin UV tubes
- Easy programming of application rates and time
- Preset nad manual controls for ultraviolet or time exposures
- Large LED display and touch panel
- Window on the door allows viewing of the process yet blocks out the UV radiation
Model CX-2000
- Similar to CL-1000 but including drawer
- Dimensions:
Exterior (D x W x H): 348 x 396 x 222 mm
Interior (D x W x H): 252 x 254 x 77 mm
Model Translinker CL-1000 UV
- Combination of Crosslinker CL-1000 and Benchtop Transilluminator M-20E (302 nm)
- Filter size 20 x 20 cm
Models
| Wavelength | Item-no. 115 V | Item-no 230 V | |
|---|---|---|---|
| CL-1000 | 254 nm | 95-0174-01 | 95-0174-02 |
| CL-1000 M | 302 nm | 95-0230-01 | 95-0230-02 |
| CL-1000 L | 365 nm | 95-0228-01 | 95-0228-02 |
| CX-2000 | 254 nm UV | 95-0339-01 | 95-0339-02 |