The product group "DNA Amplification + Cycler" contains the products AmpliCell, AmpliGrid, AmpliSpeed, ...
Conventional cell culture, treatment and extraction for gene expression analysis may induce cellular stress responses which affect gene expression. In addition, current methods require high volume culture flasks and spin-column extractions which are time-consuming and costly. The innovative AmpliCell system, which contains the AmpliCell slide and AmpliSpeed slide cycler, integrates culturing, treatment and PCR on a single platform without extraction. Minimize cellular stress and save time and reagents for transfection, microRNA assays, and methylation analysis.
Details
- On-slide cell culture of adherent cells and cells in solution
- No cell preparation needed => minimized cellular stress
- in situ cell experiments to PCR in few simple steps
- Full visual control of workflow
- 4 PCR replicates per cell culture well
- Save up to 90 % reagent costs
- Comparatible to HT systems (e.g., FACSTM, robotics)
AmpliGrid AG 480F
1 µl reaction slide
- Single Cell Analyse
- Maximale Sensitivität
- Precise Control
- Easy Handling
- DNA/RNA-free
Advalytix is introducing a superior amplification platform for ultra low volume applications in the 1µL range, based on a chemically structured microscope slide. With this innovative system, sensitivity, efficiency and reproducibility are reliable constants for single cell analysis, low-copy number approaches and ultra low volume amplification techniques. And as a result fast and convenient workflow is assured.
The AmpliGrid system is the key to obtaining perfect results especially with single cells and nearly any other kind of template material e.g., forensic stains or purified nucleic acids. With the AmpliGrid system, Advalytix is setting a new standard for low volume and single cell assays. And when we say single cell, we mean just ONE single cell.
Advanced technology for excellent ease-of-use and efficiency
The AmpliSpeed slide cycler is an ultra-compact instrument specifically designed for performing thermal cycling reactions on glass slides. An optimal thermal coupling with the glass slides is guaranteed using a superior heat transfer technology based on highly-planar silicon wafers. This unique system generates extremely homogenous surface temperatures with effective heating and cooling rates of up to 3°C/sec. This can definitely be classified as HIGH-SPEED.
Applications:
- Rt PCR single cell
- si RNA assays
- transfection assays
- high throughput gene expression analysis
Advantages:
- Ultra fast
- Superior homogeneity
- Modular system
- Independent operation
- Automation friendly
- Smallest footprint